Under the direct supervision of the Principal Investigator, the research technologist performs a variety of both routine and complex research laboratory procedures in accordance with established standards and protocols. This individual will be expected to acquire a level of knowledge to properly perform experiments and analyze results, especially where results are abnormal or atypical. Specific duties will vary according to project assigned.
Performs a variety of research laboratory techniques with primary focus on endothelial and immune cell biology related to the study of critical illness and pneumonia.
Techniques to be performed include, but are not limited to: cell culture (including primary cells and/or stem cells), PCR, cloning, sequencing analysis, and gel electrophoresis.
In addition, projects will involve DNA and RNA isolation from mouse tissue samples.
Other responsibilities include.
Preparing and determining suitability of solutions and reagents following defined formulas and procedures.
Documenting and reporting all experimental results.
Maintaining and troubleshooting equipment, routine repairs or notifying service personnel when more complex problems occur.
Performing related general duties such as ordering supplies, general lab housekeeping, and maintaining safety records.
Bachelor’s degree in biology, chemistry or related field required.
Some familiarity with basic post-undergraduate laboratory research techniques is necessary.
Special Knowledge, Skills, and Abilities:
This work requires precision and attention to detail.
Individual should have strong communication and interpersonal skills.
Must have the ability to read and interpret direction, both oral and written, organize and prioritize work and follow through on assignments.
Must be able to work successfully as part of a team in a dynamic laboratory.
Technical qualifications or specialized certifications:
Expertise in handling small rodents (mice and rats) for experiments.
Performing survival and non-survival surgeries in mice.
Harvesting organs for analyses.
Assisting with immune cell isolation procedures and cell staining for flow cytometry; performing behavioral testing in rodents using standard neurobehavioral tests; performing RNA isolation and qPCR to assess gene expression levels; and participating in the design of research studies.
Independently compile and analyze research data using various relevant statistical computer software applications.
Coordinate and maintain data reports and database files on progress of research.
May receive, inventory, enter, and organize collected data.
Significant contributions which lead to authorship on publications and oral/poster presentation of novel data will be highly encouraged.
Classified title: Research Technologist
Working title: Research Technologist
Role/Level/Range: ACRO 40/E/02/CC
Starting Salary Range: $14.14 - $19.44 (commensurate with experience)
Department name: 10002817-SOM DOM Pulmonary and Critical Care Medicine
Personnel area (School): SOM – School of Medicine
The successful candidate(s) for this position will be subject to a pre-employment background check.
If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the HR Business Services Office at email@example.com. For TTY users, call via Maryland Relay or dial 711.
The following additional provisions may apply depending on which campus you will work. Your recruiter will advise accordingly.
During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.
The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.
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