Primary focus in the Nagy laboratory is to study epigenomics of cell type specification and the role of macrophages in tissue regeneration, metabolism and tumor growth and metastasis. The individual in this role will assist in experiments in these research areas and help with general lab needs. The Research Specialist is expected to have some knowledge of cell culture and basic molecular biology techniques (PCR, Western blot, molecular cloning). This position will report directly to the lab manager for day-to-day operations.
Performs routine lab duties such as preparing buffers and stocking lab supplies.
Perform genotyping on tissue samples.
Perform qPCR or western blot experiments on cell or tissue samples.
Carry out in vitro cell culture.
Assist other lab members in preparing or carrying out similar experiments.
Drives individual research projects and contributes to other projects in the lab.
Troubleshoots and optimizes research methods and the development of new techniques.
Collects and processes data. Interprets results.
May contribute to writing manuscripts and grants methodology sections and or/ processes analyzed data into figures for scientific publications.
Maintain and organize the mouse colony including animal protocols.
Bachelor's degree in biology, chemistry or related field required.
One (1) year of prior related experience in biomedical research laboratory performing relevant technique required.
Master's degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula.
JHU Equivalency Formula: 18 graduate degree credits (semester hours) with related graduate research may substitute for one year of experience.For jobs where equivalency is permitted, up to two years of non-related college course work may be applied towards the total minimum education/experience required for the respective job.
Special Knowledge, Skills, and Abilities:
Molecular biology techniques such as DNA and RNA isolation, PCR, agarose gel electrophoresis, qPCR, and western blotting.
Exposure to toxins and infectious agents exist, but potential for personal injury or harm is minimized if established safety and health precautions are followed.
Must refer to and are expected to comply with procedure manuals, follow proper laboratory protocols and safety policy/procedures, and be familiar with material data safety sheets in assigned work areas.
Able to read and understand all Health, Safety and Environment (HSE) guidelines applicable to assigned work area.
Working in laboratory where there may be discomforts due to odors, noise, temperature fluctuations, and working around lab equipment.
Research areas may include animal, human, human products, DNA, and radiation protocols.
Use of personal protective equipment may be advised or required.
May transport equipment and supplies usually less than 40 pounds from one area to another (in the laboratory).
May be required to lift and/or move up to 50 pounds with proper training, or precautions/lifting aides (example: supply boxes).
In some areas work space is limited.
Classified title: Research Specialist
Working title: Research Specialist
Role/Level/Range: ACRP 40/E/03/MA
Starting Salary Range: $16.13 - $22.16 (commensurate with experience)
Employee group: Full time
Employee Status: Non-Exempt
Schedule: Monday-Friday - 8:30am-5:00pm 40hrs/Wk - Work hours may vary depending on experiment needs
Location: 3rd Floor - 600 5th Street South, St. Petersburg, FL 33701
Department name: 60002426-All Childrens - Nagy Laboratory, Institute for Fundamental Biomedical Research
Personnel area (School): SOM – School of Medicine
The successful candidate(s) for this position will be subject to a pre-employment background check.
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