Rutgers, The State University of New Jersey is seeking a Temporary Research Assistant for the Child Health Institute at the Rutgers Robert Wood Johnson Medical School.
Under the direction of the Principal Investigator (PI), the Temporary Research Assistant will be responsible for the performance and analysis of experiments focused on the genetic causes of congenital muscular dystrophy (CMD) associated with brain malformations and intellectual disability. The project is part of a larger effort in the lab to discover novel disease causing mutations for CMDs and to develop zebrafish models to screen therapeutic approaches. The TRA should have a broad (college-level) knowledge of genetics, molecular biology, and biochemistry and some research experience in either human genetics or zebrafish models. The TRA will be responsible for the generation, maintenance, and analysis of zebrafish models of CMDs using CRISPR/Cas9 technology and for DNA and RNA extraction from human samples.
Among the key duties of this position are the following:
Use molecular biology approaches to develop genome editing reagents using CRISPR/Cas9 to generate zebrafish mutants.
Perform microinjections to either knock-down or knock-out genes of interest in zebrafish oocytes.
With assistance from the zebrafish facility, maintain mutant colonies by breeding and genotyping.
Extract DNA and RNA from human samples when necessary and participate in the analysis of genetic data and human genetics research.
Assist in the instruction and coordination of undergraduate students in laboratory techniques and procedures in cell biology and zebrafish husbandry to carry out research assignments.
Compile and analyze data with appropriate statistical analysis, conclusions and future directions in support of further CHI research.
Keep clear and detailed records of procedures and experiments in accordance with laboratory guidelines, maintain data in shared and readily accessible locations, present data to the PI and other lab members.
Minimum Education and Experience:
Recent college graduate with B.A. or B.S. in Biology, Molecular Biology, Genetics or Neuroscience.
Required Knowledge, Skills, and Abilities:
Must be familiar with basic molecular biology procedures in a research laboratory setting.
Must have effective oral and written communication skills.
Must be computer literate with proficiency and working knowledge of database and reporting tools such as Microsoft Word, Excel, and PowerPoint.
Must have the ability to work collaboratively and independently.
Must have the ability to manage a small group of researchers.
Prior laboratory experience working with zebrafish or expertise in analyzing next-generation sequencing datasets.
Physical Demands and Work Environment:
Must be able to perform laboratory research.
Must be able to work with zebrafish.
Some lifting may be required: laboratory items, tanks, and/or boxes.
Walking, standing, sitting is required.
Salary: $16.50 - $21.00
Posting Number: 19ST3963
Location: Downtown New Brunswick
Internal Number: 99952
About Rutgers University
Rutgers, The State University of New Jersey, is a leading national public research university and the state's preeminent, comprehensive public institution of higher education. Rutgers is dedicated to teaching that meets the highest standards of excellence; to conducting research that breaks new ground; and to turning knowledge into solutions for local, national, and global communities. As it was at our founding in 1766, the heart of our mission is preparing students to become productive members of society and good citizens of the world. Rutgers teaches across the full educational spectrum: preschool to precollege; undergraduate to graduate and postdoctoral; and continuing education for professional and personal advancement. Rutgers is New Jersey's land-grant institution and one of the nation's foremost research universities, and as such, we educate, make discoveries, serve as an engine of economic growth, and generate ideas for improving people's lives.